An Analysis of the Relationship between Water Quality Indicators and Public Health in Vilnius
Vilniečių sergamumo ir geriamojo
vandens kokybės
rodiklių ryšio analizė
By Randolph B.
Flay, Fulbright Fellow &
Irena Taraškevičiene,Vilnius Public
Health Center
Abstract/Reziume:
Tirdami geriamojo vandens kokybės įtaką gyventojų
sergamumui, Vilniaus miesto teritoriją suskirstėme į 9 rajonus
ir palyginome vandens kokybinius rodiklius 1992-1995m. bei 0 – 19m. amžiaus
gyventojų sergamumo lygius 1991-1995 m. ir visų gyventojų
1991-1992 m. Ieškojome statistiškai patikimo ryšio tarp geriamojo vandens
drumstumo, kietumo, geležies, nikelio ir švino koncentracijų, bendrosios
organinės anglies kiekio ir sergamumo skrandžio, odos, inkstų ligomis,
žarnyno infekcijomis, apsigimimais. Nustatėme, kad drumstumas tiesiogiai
įtakoja sergamumą gastritu, funkciniais skrandžio sutrikimais bei
žarnyno infekcijomis vaikų ir suaugusių tarpe. Ryšio tarp geriamojo
vandens kietumo ir sergamumo inkstų akmenlige nenustatėme. Bendrosios
organinės anglies kiekis neįtakojo sergamumo žarnyno infekcijomis.
Nustatyta koreliacija tarp geležies koncentracijos vandenyje ir sergamumo
odos ligomis, švino ir apsigimimų vaikų (iki 19m.) tarpe, nikelio ir
sergamumo odos ligomis visų gyventojų tarpe.
Vilniaus centralizuotai tiekiamo vandens drumstumas
koreliuoja su geležies koncentracija.
Introduction:
Via
the many ways that we contact the natural environment, perhaps no relationship
is greater than that between humans and water.
We use it for the preparation of foods, personal hygiene, and often
consume directly 2-3 liters per day, much stemming directly from our taps.
Further increasing its importance and necessitating its protection is the
vulnerability of groundwater to contamination from anthropogenic land-use
practices and the high cost of remediation in such an event. Given this strong
dependency on water, it is important to understand how water affects our health
and ensure that it is properly protected and managed.
Nineteen
well fields in Vilnius supply approximately 155,000 cubic meters per day to the
citizens of Vilnius, comprising the bulk of drinking water consumed as shown in
Map 1. These 19 well fields supply the municipal water supply system and
can be broken down into 9 sections, see
Table 1. Since people drink water
from these 9 water supply systems and not directly from the well field, any
study looking at public health should look at water quality and public health
by the geographic location of these water supply systems.
In
this brief and preliminary study, we examined public health data in Vilnius
from years 1991 to 1995 in conjunction with sets of water quality data spanning
1992-1995. Our first goal was to look
at the public health data geographically to see how disease rates vary
throughout the city. The second goal
was to examine the water quality data and see if any of the variability in the
disease rates could be explained by the available water quality data based on
documented relationships from epidemiological studies.
Most importantly, this study should demonstrate the integrated nature of issues such as drinking water quality and the need for continuous information exchange among agencies that work in this field in Vilnius. With effective and timely information exchange, agencies can hopefully make better informed and therefore more effective decisions regarding the management of drinking water in the city.

Data and Methods:
Public Health Data
Public health data were obtained from the public health database maintained by the Vilnius Public Health Center or Vilniaus Visuomenes Sveikatos Centras (VSC). Each time a patient visits a polyclinic in Vilnius, a record is sent to the VSC recording the diagnosis, the patient’s age, residence, the date, and other useful information. The data spanned the years 1991 to 1995 for ages 0-19 and years 1991 and 1992 for all ages. After 1992, only records for those under age 20 were kept. The data set contains over 1 million records and diseases are recorded utilizing the International Classification of Diseases, 9th Revision (ICD) codes (Министерство здравоохранения СССР).
Water Quality Data
The water quality data came primarily from the VSC that performs water quality testing in the municipal water supply system throughout Vilnius for basic indicators of water quality such as total hardness, turbidity, iron, and others. This database spanned the years 1992 to 1995 and contained approximately 400 geographically classified records per water quality indicator. We favored the use of this data set over others because this data set takes measurements from the tap, i.e. the end consumption point, and thus should be the most representative of the quality of water people are actually consuming.
We also utilized the local water company’s, Vilniaus Vandenys’s, water quality data for nickel, lead, and turbidity levels as measured at the water supply system reservoirs. The reservoirs are typically located at a point where water from separate well fields combines and is treated before entering the distribution system to consumers.
Twenty-six Total Organic Carbon (TOC) samples were also taken throughout the city as part of a pilot project in March 1999 and analyzed at labs in Siauliai and in Chicago.
Analysis
Raw data from the VSC was collected in a DB4 database format. In order to analyze the information, a Microsoft Access database was designed and the information imported. Also developed in the database was a feature to analyze the data geographically by water supply system. To accomplish this, each street in both the public health and water quality databases was assigned to the water supply district in which it is located. This allowed for the creation of queries based on the water supply systems; i.e. one could then calculate the number of diagnoses of a particular illness or the average level of a water quality indicator by water supply system. All streets where also classified by the 20 city districts.
Utilizing a data table that also included the population living on each street in Vilnius, annual disease rates were calculated per 100,000 residents. In total we composed two public health data sets; one contained all ages for years 1991 to 1992 and the second contained ages 0-19 but spanned from 1991 to 1995. (This is again because health data after 1992 was only collected for children). Since to analyze the data for years 1991 to 1995 we only are looking at patients aged 0-19, we had to multiply by the portion of the population represented by the age-group 0-19. This was roughly calculated as 0.261 (Vilnius Statistical Office).
After calculating disease rates and the average of water quality results in each of the nine distribution systems, we began to look for relationships between the levels of water quality indicators and the levels of various diseases. Our searches were aided through documented relationships in medical and scientific journals on the relationships between water quality and public health. For more detailed statistical analyses, discrete data sets were exported from the Microsoft Access database to Microsoft Excel and to Systat 7.0 for Windows.
With a data set of water quality data and public health data, we performed a linear regression analysis holding the water quality indicator as independent and the disease rate as the dependent. We also regressed certain sets of water quality data to other sets of water quality data, such as the relationship between turbidity measured at water supply reservoirs and turbidity measured at taps. The details and results of each analysis can be seen in Table 5.
Results:
Description of Water
Quality Results
The water quality indicators utilized in this study include turbidity, total hardness, TOC, nickel, lead, and total iron. Table 2 contains the water quality data set and detailed information about how, when, and where sampling took place. Table 3 contains descriptive statistics for the water quality data.

The
ICD codes that we reviewed include 001-009 (Intestinal Infections), 535 (Gastritis),
536 (Disorder of Stomach Functions), 592 (Kidney Stones), 690
(Erythematosquamous dermatosis), and 740-759
(Growth-related disorders). Table 4 contains the disease rates
calculated for each water supply system for each selected ICD codes.

Description of the Relationships between
Water Quality Indicators and Diagnosis Rate Results

Also included in Table
5 is the p-value of the relationship.
The p-value is a measure of the significance of the relationship in the
sense that the samples we have taken are representative of the total
population. The p-value is essentially
based on the sample size, a larger sample size being more representative of the
whole population. A p-value of 0.093
means that there is a 9.3% chance that the relationship that we have found
between the variables is a complete “fluke.”
For the purpose of this study we have chosen a p-value < 0.10 for
values that we will accept, which corresponds to an α > 90%. Although the R2 value and the
p-value are related, one often refers to the R2 value as a measure
of strength or magnitude and the p-value as a measure of significance.
It
can be noticed that we did find several statistically significant and strong
relationships as displayed in Table 5.
For the age group 0+ years and the 1991-1992 data set, we noticed a few
interesting findings. The relationship
between turbidity levels and gastritis (ICD 535) had an R2 value of
0.351 and a p-value of 0.093, again meaning that the relationship between the
variables explains 35.1% of the variance in the variables. Other strong relationships included the
relationship between nickel levels and erythematosquamous dermatosis (ICD 690)
with an R2 value of 0.719.

We also examined total
hardness to kidney stones (ICD 592) and TOC to intestinal infections (ICD
001-009) but the relationships showed below acceptable p-values. As a further note on the TOC measurements,
this was the first time the samples were taken in Vilnius and we believe that
further samples should be taken before extensively comparing this indicator to
public health. There were very few TOC
samples taken and the analyses of identical samples at two labs did not
coincide. Further, both microbiological
activity and organic chemicals can contribute to TOC levels, and in the absence
of data pertaining to the percentage breakdown of each contaminant, it is very
difficult to say how TOC levels would be manifest in public health data
(Greenburg).
In
the age group of 0-19 years for 1991-1995, we also found some interesting
relationships. Turbidity related to
gastritis (ICD 535) and disorders of stomach function (ICD 536) with R2
values of 0.602 and 0.603. Average
total iron measurements taken in both municipal and private water systems related
to erythematosquamous dermatosis (ICD 690) with an R2 value of
0.617. Average lead levels taken at
water supply reservoirs yielded an R2 value of .400 in relation to
growth-related disorders (ICD 740-759).
We
also looked at turbidity levels recorded at reservoirs versus those at taps,
yielding an R2 value of 0.544 and total iron to turbidity, yielding
an R2 value of 0.854.
Discussion:
The relationship between turbidity and gastrointestinal illness is well-documented in epidemiological studies (Schwartz). Although turbidity alone may not represent a health threat, increased turbidity levels create a medium for microbiological activity and can compromise disinfection treatments. Turbidity may also act in other ways affecting public health, but the influence is not clear. Microbiological activity should specifically be manifest in a relationship to intestinal infections (ICD 001-009), but may also turn up other gastrointestinal disorders such as gastritis (ICD 535) and disorder of stomach function (ICD 536). As noted in Table 5, we confirmed many of relationships in the drinking water situation in Vilnius.
Increased nickel levels have also been proven to increase incidence of disease affecting the skin, stomach, blood, liver, kidneys, immune system, and reproduction and development in rats and mice (Agency for Toxic Substances and Disease Registry). Given this research our findings of increase incidence of erythematosquamous dermatosis (ICD 690) coinciding with increased nickel levels could be quite notable.
Developmental and growth-related disorders in children have also been associated with increased levels of lead intake (Agency for Toxic Substances and Disease Registry). Our study also found a relationship between lead levels and growth-related disorders in children (ICD 740-759).
Although a scientific study has yet to prove that increased iron levels in water can contribute to skin-related disease, our results did show a strong connection to erythematosquamous dermatosis (ICD 690). Further research should attempt to see if this is true. Also in association with iron levels, we noticed a very strong correlation between iron levels and turbidity levels. If iron contributes to turbidity and turbidity leads to increases in gastrointestinal illness, then negative health effects are stemming indirectly from iron.
The relationship between turbidity as measured in reservoirs and turbidity as measured from taps seems quite logical. However, we should note that turbidity levels were substantially higher as measured from the tap. This situation highlights the need for coordination between source and terminus water quality measurements. Water quality monitoring at the source is necessary, but to the consumers the most important location for monitoring is of course at the tap.
Lithuanian Hygienic Norm 1998 Drinking Water has set new standards for water quality and the law will be phased in, beginning in the year 2000. It should be noted that maximum contaminant levels (MCL) for nickel as set in this new law are frequently exceeded in Vilnius water supply systems. Lead levels, turbidity levels, and iron levels are also found quite often in above MCL quantities based on this new law. Table 6 outlines some of the potential compliance problems. Given the known health impacts of these contaminants in drinking water Vilnius should consider ways bringing water quality into compliance with national laws.

It is important to mention some of the potential sources of error in this
experiment. In an analysis of a water
quality indicator such as nickel, levels may increase in series with other
contaminants in water. Although levels
of heavy metals will vary little over a short time period, turbidity levels
fluctuate regularly and using a 4-year average might not be the most effective
method for measuring short-term fluctuations in turbidity. Unlike heavy metals, which typically will
have a cumulative effect on health, turbidity will likely be manifest in
short-term health effects. Given that
we were performing an analysis over 9 discrete populations, the 9 water supply
districts, other environmental or social factors could also be at work
influencing our results.
Conclusion:
The
first goal of the study was to look for differences in disease rates in Vilnius
geographically by water supply district and see if any of the differences could
be explained based on available water quality data. We did find substantially divergent disease rates across a wide
variety of disease groupings. This
definitely indicates that there is a strong environmental or social influence
at work.
The
second goal was to identify the cause of the variance. Although preliminary studies such as this
cannot be the final, definitive word, we think the findings here suggest that
water quality is having an impact on health.
The importance research is further stressed by the published health effects of these
contaminants and the nonsuccess of Vilnius to obtain national hygienic norms
set for these contaminants as outlined in Table 6.
To
summarize our major findings:
·
Turbidity
positively related to gastrointestinal illnesses (ICD 535 & 536) in the
young 0-19 year group and in the 0+ year group.
·
Lead
levels positively related to growth-related disorders (ICD 740-759) in the 0-19
year age group.
·
Nickel
levels positively related to erythematosquamous dermatosis (ICD 690) in the 0+
year age group.
·
Total
iron levels positively related to erythematosquamous dermatosis (ICD 690) in
the 0-19 year age group.
In
the near future we would recommend a time-series analysis of turbidity and
microbiological data in conjunction with public health data for
gastrointestinal illness. By performing
a time-series analysis on one study group, many social and other environmental
factors will diminish in influence and allow the study to focus more
specifically on the effects of a given water quality indicator such as
turbidity.
Studies
such as these will also be aided with more liberal agency policies regarding
data sharing. Although the public
health data for Vilnius are maintained in a single location, several groups,
such as the VSC, Vilniaus Vandenys, and consulting firms, maintain separate
databases for water quality data. This
diminishes the value of the data and makes comprehensive analyses difficult. Further the Municipality should look to see
what other indicators need to be monitored given the natural and anthropogenic
load on Vilnius well fields. Monitoring
of organic chemicals and other microbiological conditions would seem
appropriate in a city the size of Vilnius.
Most important, there is substantial variance in disease rates among
water supply and city districts, and a geographic approach has proved
successful in examining public health data by water supply systems can be very
helpful in identifying health problems stemming from water quality.
References:
Agency
for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for lead. Atlanta, GA: U.S.
Department of Health and Human Services, Public Health Service, 1993.
Agency
for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for nickel (update). Atlanta, GA:
U.S. Department of Health and Human Services, Public Health Service, 1996.
Greenberg,
Arnold E. and others. Standard
Methods for the Examination of Water and Wastewater. 18th Edition. 1992.
Klimas, Algirdas and others. Vilniaus Miesto Vandenviečių Eksploatacijos ir
Vandens Tiekmo Sistemos Optimizacija. SPUAB "Vilniaus Vandenys"
ir UAB Vilniaus Hidrogeologija. Vilnius: Vilniaus Hidrogeologija, 1998.
Schwartz,
Joel and others. Drinking Water
Turbidity and Pediatric Hospital Use for Gastrointestinal Illness in
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Statistical
Office of Vilnius. Vilnius in
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Apskrities Statistikos Valdyba, 1998.
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